Figure 7.

GCAWKR Regulates GC Development by Mediating PTP4A1 Expression

(A) CCK-8 assays showed that cell proliferation was inhibited in GCAWKR-overexpressing BGC823 cells after the cells were transfected with siRNA-PTP4A1. (B) Colony-formation assays showed that colony-formation ability was suppressed in GCAWKR-overexpressing BGC823 cells after the cells were transfected with siRNA-PTP4A1. (C) Effects of PTP4A1 knockdown on cell invasion in GCAWKR-overexpressing BGC823 cells in the presence of the anti-proliferative drug mitomycin C (MMC, 5 μM) treatment were determined using the transwell assay. *p < 0.05. (D) Top left, representative images of tumors formed in nude mice injected subcutaneously with BGC823 cells transfected with desired vector. Top right, tumor weights. Middle left, tumor growth curves. Bottom, representative images of IHC staining of Ki67 (original magnification, ×200). *p < 0.05.