Figure 4.

lncMUMA, but Not Mutated lncMUMA, Counteracted the Decreases in MyoD Protein Level and Myotube Formation in C2C12 Cells Stably Overexpressing miR-762 In Vitro

(A) (Left) Western blot analysis of MyoD protein in C2C12 cells stably overexpressing miR-762 transfected with empty vector (EV), lncMUMA vector, and mutated lncMUMA vector (lncMUMA-Mut), respectively, on days 0, 1, 3, 5, and 7 of differentiation. (Right) Representative images of MyoD protein in each group on day 7. (B) Representative images of C2C12 cells in each group on day 7 of differentiation. Myosin was labeled with green fluorescence and the nuclei were labeled with DAPI. Scale bars, 50 μm. (C) The fusion index in C2C12 cells in each group on day 7 of differentiation. n = 5 at each time point for each group. β-actin is used as the control for protein. Data are presented as mean ± SEM. *p < 0.05 versus the corresponding day 0.