Figure 6.

Enforced lncMUMA Expression Could Counteract the Decreases in MyoD Protein Level and Myotube Formation in Microgravity-Simulated C2C12 Myoblasts In Vitro

(A) Real-time PCR analysis of lncMUMA levels in C2C12 cells transfected with either lncMUMA overexpression vector (lncMUMA) or empty vector (EV) under microgravity-simulated (MGS) culture environment on days 0, 1, 3, 5, and 7 of differentiation. (B) Western blot analysis of MyoD protein level in C2C12 cells transfected with lncMUMA or EV under MGS culture environment on days 0, 1, 3, 5, and 7 of differentiation. (C) Representative images of C2C12 cells transfected with lncMUMA or EV under MGS culture environment on day 7 of differentiation. Myosin was labeled with green fluorescence and the nuclei were labeled with DAPI. Scale bars, 50 μm. (D) The fusion index in C2C12 cells transfected with lncMUMA or EV under MGS culture environment on day 7 of differentiation. n = 5 at each time point for each group. U6 small nuclear RNA is used as the internal control of lncRNA. β-actin is used as the control for protein. Data are presented as mean ± SEM. *p < 0.05 versus the corresponding day 0.