Table 3.
Linearity, LOD, LOQ, precision, repeatability, stability and recovery of the 12 chemical markers.
| Chemical Markers | Regression Equation | Linearity (μg/mL) | LOD (μg/mL) |
LOQ (μg/mL) |
r | Precision RSD (%) |
Repeatability RSD (%) |
Stability RSD (%) |
Recovery (mean ± SD) |
|---|---|---|---|---|---|---|---|---|---|
| Eriocitrin | Y = 17.787X + 0.7195 | 0.19–39.00 | 0.06 | 0.15 | 0.9999 | 1.12 | 0.93 | 3.17 | 101.35 ± 1.02 |
| Neoeriocitrin | Y = 14.754X - 3.5435 | 0.38–190.00 | 0.09 | 0.33 | 0.9995 | 1.65 | 1.87 | 3.38 | 101.89 ± 0.33 |
| Narirutin | Y = 25.375X + 13.516 | 0.41–205.00 | 0.11 | 0.40 | 0.9998 | 1.56 | 0.84 | 0.19 | 102.36 ± 0.21 |
| Naringin | Y = 18.107X + 9.3102 | 0.44–880.00 | 0.12 | 0.38 | 0.9998 | 1.97 | 0.89 | 0.21 | 100.98 ± 0.87 |
| Hesperidin | Y = 15.396X + 3.6443 | 0.74–740.00 | 0.21 | 0.67 | 0.9997 | 1.40 | 0.83 | 0.20 | 102.95 ± 1.21 |
| Neohesperidin | Y = 26.069X + 10.197 | 0.42–840.00 | 0.10 | 0.39 | 0.9999 | 1.23 | 0.83 | 0.09 | 101.05 ± 1.92 |
| Poncirin | Y = 17.126X + 5.1686 | 0.11–88.00 | 0.03 | 0.09 | 0.9995 | 0.87 | 0.72 | 0.26 | 100.77 ± 0.65 |
| Naringenin | Y = 37.591X + 5.2889 | 0.06–22.00 | 0.02 | 0.06 | 0.9999 | 0.86 | 1.41 | 0.26 | 98.35 ± 0.55 |
| Hesperetin | Y = 47.777X + 4.2667 | 0.06–22.50 | 0.02 | 0.06 | 0.9999 | 0.83 | 1.99 | 1.98 | 99.07 ± 0.73 |
| Nobiletin | Y = 38.995X + 2.8379 | 0.20–39.00 | 0.05 | 0.18 | 0.9999 | 0.86 | 2.58 | 2.23 | 102.22 ± 1.24 |
| Tangeretin | Y = 41.882X + 5.1345 | 0.19–38.00 | 0.05 | 0.17 | 0.9999 | 0.90 | 3.50 | 0.38 | 101.99 ± 0.90 |
| Auraptene | Y = 11.111X + 1.7305 | 0.18–36.00 | 0.04 | 0.16 | 0.9999 | 0.81 | 1.13 | 1.29 | 98.36 ± 1.59 |