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The correlation between absorbance and NaHS concentration determined by the DTNB assay in sodium phosphate buffer (pH 8.0) at room temperature. The absorbance at 412 nm was recorded for 15 min after addition of various concentrations of NaHS from 20 to 100 μm. Values of absorbance are given as means obtained from 3 measurements. Background values were subtracted from the sample values.
