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. 2018 Aug 24;23(9):2134. doi: 10.3390/molecules23092134

Figure 4.

Figure 4

Gel image showing the effectiveness of the multiplex SCAR assay on DNAs of individual Angelica species (controls) and mixed template DNAs prepared by mixing the templates of two or more species. PCR products were produced using three primer pairs (ADA-F/ADA-R, AAN-F/AAN-R, and AJA-F/AJA-R) in a single PCR reaction. The template DNA used in each lane is indicated. Lane M represents a 100 bp DNA ladder. Arrows indicate the sizes of PCR products, and arrowheads indicate the sizes of different molecular weight bands of the DNA ladder.