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. 2018 Aug 21;23(9):2097. doi: 10.3390/molecules23092097

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) The bacterial growth curve (OD/hours). All the strains of S. aureus (ATCC BAA-977, ATCC 25923, ATCC 6538) and E. coli (ATCC8739) were grown into the reference medium (MHII for the ATCC BAA-977 strain and MHI for the others strains). The starting inoculum was about 5 × 10⁵ CFU/mL. The growth abilities were evaluated by optical density (OD) determination during the time (from time = 0 to time = 24 h). (B) Bacterial growth curve (CFU/hours). The same starting inoculum of each strain was used to analyze the unit forming colony (CFU/mL) during the time (from time = 0 to time = 24 h) in the same conditions used for the growth curve determination. Both experiments were done in triplicate to calculate the median and standard deviation by the GraphPad Prism6 software.