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. 2018 Aug 21;23(9):2097. doi: 10.3390/molecules23092097

Figure 4.

Figure 4

The time kill curve assay. MOR/α-CD bactericidal activity was assessed against S. aureus ATCC BAA-977, ATCC 25923, and ATCC 6538 strains using the MIC dose (0.5 mg/mL) of MOR/α-CD and from 2 up to 4-fold the value of MIC for each strain (0.5, 1 and 2 mg/mL). The colony count was made after 18 h, 20 h, and 24 h of treatment. Experiments were assessed in triplicate to obtain the mean and the standard deviation (SD). A statistical analysis (Two way ANOVA) was performed by the GraphPad Prism6 software and the p value < 0.0001 (****) showed a significant difference between the dose of 0.5 mg/mL, 1 mg/mL, and 2 mg/mL of MOR/α-CD and the untreated bacteria (0 mg/mL) for each strain at different time points.