Table 3.
Specific activities of transamination enzymes with leucine and valine as substrates.
| Strain | Growth Conditions a | Aminotransferase Specific Activity (mU/mg of Protein) |
|
|---|---|---|---|
| Leucine | Valine | ||
| FA-1△ilvE△aspB/pEC-XK99E-tyrB | +Ile + Val + Leu + Asp | 16.85 ± 1.19 | <1 |
| FA-1△ilvE△aspB/pEC-XK99E-ybgE | +Ile + Val + Leu + Asp | 16.11 ± 2.01 | 8.79 ± 1.33 |
| FA-1△ilvE△aspB/pEC-XK99E-CaIlvE | +Ile + Val + Leu + Asp | 15.94 ± 1.88 | 8.33 ± 1.28 |
| FA-1△ilvE△aspB/pEC-XK99E-aspC | +Ile + Val + Leu + Asp | <1 | <1 |
| FA-1△ilvE△aspB/pEC-XK99E-yhdR | +Ile + Val + Leu + Asp | <1 | <1 |
| FA-1△ilvE△aspB/pEC-XK99E-ywfG | +Ile +Val + Leu + Asp | <1 | <1 |
a The medium CGXIIG contained l-methionine. All data represent values of three determinations of three independent experiments with ± SD.