Table 4.
Strains and plasmids used.
| Strains and Plasmid | Description | Source or Reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| BL21(DE3) | F- ompT gal dcm lon hsdSB (rB-mB-) λ(DE3) | Strata gene |
| W3110 | Wild type | Lab stock |
| C. glutamicum | ||
| ATCC 13032 | Type strain | ATCC |
| FA-1 | ilvE + aspB + | Lab stock |
| FA-1△ilvE | As in FA-1, ilvE- | This work |
| FA-1△ilvE/pEC-XK99E-aspB | As in FA-1△ilvE, aspB+ | This work |
| FA-1△ilvE△aspB | As in FA-1△ilvE, aspB- | This work |
| FA-1△ilvE△aspB/pEC-XK99E-tyrB | As in FA-1△ilvE△aspB, tyrB+ | This work |
| FA-1△ilvE△aspB/pEC-XK99E-ybgE | As in FA-1△ilvE△aspB, ybgE+ | This work |
| FA-1△ilvE△aspB/pEC-XK99E-CaIlvE | As in FA-1△ilvE△aspB, CaIlv + | This work |
| FA-1△ilvE△aspB/pEC-XK99E-aspC | As in FA-1△ilvE△aspB, aspC+ | This work |
| FA-1△ilvE△aspB/pEC-XK99E-yhdR | As in FA-1△ilvE△aspB, yhdR+ | This work |
| FA-1△ilvE△aspB/pEC-XK99E-ywfG | As in FA-1△ilvE△aspB, ywfG + | This work |
| B. subtilis 168 | Wild type | ATCC |
| C. acetobutylicum ATCC 824 | Wild type | ATCC |
| Plasmids | ||
| pk18mobsacB | Integration vector | Lab stock |
| pk18mobsacB-△ilvE | pk18mobsacB carrying ilvE-L and ilvE-R gene | This work |
| pk18mobsacB-△aspB | pk18mobsacB carrying aspB -L and aspB -R gene | This work |
| pEC-XK99E | E. coli-C. glutamicum shuttle vector and Kanr | Lab stock |
| pEC-XK99E-aspB | pEC-XK99E with a 1.3 kb Kpn I/Xba I fragment containing aspB gene | This work |
| pEC-XK99E-tyrB | pEC-XK99E with a 1.2 kb EcoR I/BamH I fragment containing tyrB gene | This work |
| pEC-XK99E-ybgE | pEC-XK99E with a 1.0 kb EcoR I/BamH I fragment containing ybgE gene | This work |
| pEC-XK99E-CaIlvE | pEC-XK99E with a 1.0 kb EcoR I/BamH I fragment containing CaIlvE gene | This work |
| pEC-XK99E-aspC | pEC-XK99E with a 1.2 kb EcoR I/BamH I fragment containing aspC gene | This work |
| pEC-XK99E-yhdR | pEC-XK99E with a 1.1 kb EcoR I/BamH I fragment containing yhdR gene | This work |
| pEC-XK99E-ywfG | pEC-XK99E with a 1.2 kb Kpn I/Xba I fragment containing ywfG gene | This work |