Figure 6.

Effects of compound 2 on the expression of Nrf2 and HO-1 in MIN6 cells. (A) Expression levels of Nrf2-mediated antioxidant proteins were detected by Western blot analysis in 16 h. (B,C) Quantification of Nrf2 and HO-1 protein expression. Data are presented as means ± SDs of three independent experiments. ^& p < 0.05, ^&& p < 0.01 compared with control group (untreated with H₂O₂ and compound 2). ^## p < 0.01, ^### p < 0.001 compared with model group (only treated with H₂O₂). (D) Expression levels of Nrf2-mediated antioxidant proteins after the cells were incubated with 100 μM of compound 2 for the indicated time period. (E,F) Quantification of Nrf2 and HO-1 protein expression. Data are presented as means ± SDs of three independent experiments. ** p < 0.01, *** p < 0.001 compared with control group.