Figure 5.

(left) Fluorescence (ex: 809 nm, em: 829 nm, slit 5 nm) titration isotherm for incremental addition of M2 to a solution of S3PEG (250 nM) in H₂O at 25 °C. (right) Threading kinetic profile generated by mixing equal molar concentration (50 nM each) of S3PEG and M2 in a stopped flow device (ex: 809 nm, em: 829 nm, slit 5 nm). The red lines are computer fits of experimental data to 1:1 binding model or second order kinetic model, respectively.