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. 2018 Sep 3;23(9):2236. doi: 10.3390/molecules23092236

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of PA on E-cadherin and α-SMA expression in UUO mice. (a) Immunoblot results show the effects of PA on the inhibition of UUO-induced changes in EMT markers, including E-cadherin and α-SMA. β-Actin was used to confirm equal sample loading. (b) Immunofluorescence double staining for E-cadherin (green) and α-SMA (red) localization. Cells were counterstained with Hoechst 33342 (blue). Scale bar 100 μm. The data are representative of three independent experiments and quantified as mean values ± SEM. Tukey’s multiple comparison test, * p < 0.05, ** p < 0.01, *** p < 0.001 compared to control.

Effect of PA on E-cadherin and α-SMA expression in UUO mice. (a) Immunoblot results show the effects of PA on the inhibition of UUO-induced changes in EMT markers, including E-cadherin and α-SMA. β-Actin was used to confirm equal sample loading. (b) Immunofluorescence double staining for E-cadherin (green) and α-SMA (red) localization. Cells were counterstained with Hoechst 33342 (blue). Scale bar 100 μm. The data are representative of three independent experiments and quantified as mean values ± SEM. Tukey’s multiple comparison test, * p < 0.05, ** p < 0.01, *** p < 0.001 compared to control.