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. 2018 Aug 21;23(9):2100. doi: 10.3390/molecules23092100

Figure 3.

Figure 3

Expression of FXYD2γa in pancreatic human islets and EndoC-βH1 cells. (A) Quantitative RT-PCR (qPCR) of FXYD2γa mRNA expression in EndoC-βH1 cells (n = 5) and human pancreatic islets (n = 4). Data are presented as a box-plot. (B) A representative immunoblot of EndoC-βH1 cells, with alpha-tubulin as a reference protein (n = 3); (C) Immunocytochemistry of EndoC-βH1 cells showing surface localization of FXYD2γa (green) and Hoechst staining of nuclei (blue) (n = 3). The negative staining control of EndoC-βH1 cells (without the FXYD2γa antibody) is presented at the right side of panel C; the white scale bar represents 1 µm. The luminosity and the contrast were uniformly increased in both pictures to improve visualization. The original pictures are shown in Figure S5 (D–I). Histological evaluation of the implanted EndoC-βH1 tumors, showing cells clustered into pseudo-islets surrounded by fibrotic tissue (D), capillary networks (yellow arrows) (E), insulin expression (F) with its corresponding negative control (G) and FXYD2γa detected with either SPY393 polyclonal antibody (H) or the biotinylated P88 (I); (D–I) are representative micrographs from 2 EndoC-βH1 cell-implanted mice.