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. 2018 Sep 11;23(9):2311. doi: 10.3390/molecules23092311

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Site-specific labeling of cpTMVP with donor (Texas Red) and acceptor (Alexa Fluor 647) chromophores to measure assembly. (a) A cartoon representation of a cpTMVP disk shows the approximate locations of donor and acceptor chromophores. Double modification does not perturb the structure of disks as measured by TEM. (b) Overlay of the emission spectra of donor only and donor-acceptor systems shows the assembly-dependent FRET. Reprinted from a previous paper [116]. Copyright 2010 American Chemical Society.