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. 2018 Sep 8;23(9):2298. doi: 10.3390/molecules23092298

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of BBR on intestinal adenoma expression of β-catenin and cyclin D1 in Apc ^min/+ mice. Immunostaining of cyclin D1 (A) and β-catenin (B) in different groups: top panel: bar = 250 μm (50 μm × 5); bottom panel: bar = 100 μm (20 μm × 5). (C) BBR downregulated the expression of cyclin D1 and β-catenin in the intestinal adenoma. (C-1) Representative results of three Western blotting repetitions. (C-2) The statistical results of three repetitions. Control: wild type mice fed with a standard diet; model: Apc ^min/+ mice that received a high fat diet; BBR: Apc ^min/+ mice that received a high fat diet and supplemented with BBR. The data are expressed as means ± standard errors. *** represents p < 0.001 compared with the indicated group.