Table 1.
IC50 values of higenamine and Trolox in various antioxidant assays.
| Assay | Higenamine μM | Trolox μM | IC50,Trolox/IC50,higenamine | |
|---|---|---|---|---|
| DPPH•-scavenging assay | 17.1 ± 0.2 a | 23.3 ± 0.3 b | 1.4 | Average 1.9 |
| PTIO•-scavenging assay (pH 7.4) | 55.0 ± 1.7 a | 81.0 ± 5.6 b | 1.5 | |
| Fe3+-reducing power assay | 30.0 ± 0.3 a | 63.1 ± 8.0 b | 2.1 | |
| Cu2+-reducing power assay | 57.0 ± 1.0 a | 154.8 ± 2.0 b | 2.7 |
IC50 value was defined as the concentration of 50% effect percentage and expressed as mean ± SD (n = 3). Means values with different superscripts (a or b) in the same row were significantly different (p < 0.05). Trolox acted as positive control. The dose-response curves and IC50 calculation are listed in Supplementary 2, 4–5. Abbreviations: DPPH•, 1,1-diphenyl-2-picryl-hydrazl radical; PTIO•, 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide radical.