Figure 1.

Alkaloid chromatograms for biomass extracts from the (a) leaf, (b) stem, and (c) root/rhizome of V. californicum. Common and unique alkaloids identified by MS are observed in each extract. Labelled peaks correspond to the data summarized in Supplemental Table 1. Additional observed peaks that are not labelled did not have molecular formulas consistent with jervine-type alkaloids. Extracted ion chromatograms (EIC) are shown in (d,g) demonstrating the presence of veratramine and cyclopamine isomers in stem and root/rhizome extracts, respectively. The total ion chromatogram is shown in (d) for the stem extract (black) and EIC (grey) generated using the m/z window 410.3023 ± 0.01. The mass spectra for the peaks indicated by * in (d) are shown in (e,f). The total ion chromatogram is shown in (g) for the root extract (black) and the EIC (grey) generated m/z window 412.3186 ± 0.02. The mass spectra for the peaks indicated by * in (g) are shown in (h–j).