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. 2018 Aug 29;23(9):2178. doi: 10.3390/molecules23092178

Figure 1.

Figure 1

ASI (25 mg/kg) increased the total number of proliferative cells (BrdU+), pre-mature neurons (DCX+) and early proliferative cells (BrdU+/DCX+). (A–I) Confocal images of BrdU (red) and DCX (green) immunostaining: (A–C) control group; (D–F) ASI group; and (G–I) enlarged images of the cells from ASI group. (A–F) Scale bar = 100 μm. (G–I) Scale bar = 2 μm. (J–L) Quantification of BrdU+, DCX+ and BrdU+/DCX+ immunopositive cells in two groups (n = 4/group). (M) Western blotting analysis of DCX in hippocampus. (N) Gray intensity analysis of DCX in hippocampus (n = 8/group). * p < 0.05. The data are presented as mean ± SD.