Table 1. Kinetic Parameters for PrP and αS Aggregation in Solutiona.
| protein | ke (M–1 s–1) | kf (s–1) | kekf (M–1 s–2) | m (nM) | t2 | PK-sen to PK-res conversion half-time |
|---|---|---|---|---|---|---|
| PrP | ∼104 b | ∼10–6 b | 0.06 ± 0.03 | 60 (PM) | 2.2 ± 1.1 h | <0.25 h |
| 15 (EC) | 4.4 ± 2.2 h | |||||
| αS | 43 ± 7 | 1.6 ± 0.2 × 10–10 | 6.9 ± 1.4 × 10–9 | 2000 (SN) | 48 ± 2 day | 39.5 ± 7.3 h |
a
ke elongation rate constant; kf fragmentation rate constant; m local concentration of monomers in the cells; t2 doubling time required for a single protein aggregate to replicate into two aggregates during aggregation. PM: plasma membrane; EC: endosomal compartments; SN: synapse. The errors represent uncertainties of the fitting parameters given the dataset.
b
Estimate is within the same order of magnitude.