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Influence of stirring rate on the conversion of valeric acid to ethyl valerate and on the specific activity of prepared biocatalysts.
| Acid-to-Alcohol Ratio | Conversion to Ethyl Valerate (mol %) | Specific Activity (U/gbiocatalyst) | ||||||
|---|---|---|---|---|---|---|---|---|
| 250 rpm | 500 rpm | 750 rpm | 1000 rpm | 250 rpm | 500 rpm | 750 rpm | 1000 rpm | |
| 1:3 | 73 | 76 | 75 | 81 | 478 ± 10 | 490 ± 11 | 497 ± 9 | 501 ± 13 |
| 1:2 | 89 | 81 | 87 | 95 | ||||
| 1:1 | 92 | 87 | 87 | 90 | ||||
Reaction conditions: 12% m/v of biosilicified enzymes, 250–1000 rpm, 40 °C, 2 h CH in batch reactor.
