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Effect of reaction time on MW and CH conducted esterification of valeric acid under the main optimised reaction conditions.
| Methodology | Conversion to Ethyl Valerate (mol %) | Specific Activity (U/gbiocatalyst) | ||||
|---|---|---|---|---|---|---|
| 15 min | 30 min | 60 min | 15 min | 30 min | 60 min | |
| MW | 80 | 80 | 82 | 394 ± 18 | 384 ± 16 | 357 ± 10 |
| CH | 86 | 90 | 90 | 545 ± 14 | 495 ± 11 | 460 ± 11 |
Reaction conditions: molar ratio 1:2 of valeric acid to EtOH, 12% m/v biosilicified enzyme, 100 W (MW), 40 °C (CH).
