Figure 3.

Ground state femtosecond-stimulated Raman spectroscopy (FSRS) of the GFP-S205V mutant with 801 nm (red), 539 nm (black), and 504 nm (blue) Raman pump in the Stokes, Stokes, and anti-Stokes experimental setup, respectively. The double-arrowed line denotes the stimulated Raman gain magnitude of 0.05%. The dashed gray line marks zero Raman gain. The green arrows highlight some marker band intensity and frequency change from the conventional FSRS (red) to tunable FSRS (black and blue traces).