Figure 5.

Astrocyte-derived exosomes can be taken up by neuronal SH-SY5Y cells. (A) Exosomes observed by transmission electron microscope; (B,C) Nanoparticle tracking analysis of exosomes; (B) The signal of scattered light by dynamic nanoparticles; the bright white dot indicates one moving particle; (C) Particle size distribution of nanoparticles; (D) Western blotting was used to detect TSG101, CD63, andCD81 proteins of exosomes. Ctl-exo: exosomes extracted from cultured primary astrocytes; mor-exo: exosomes extracted from cultured primary astrocytes treated with 100 μM morphine for 48 h; Sino-exo: exosomes extracted from cultured primary astrocytes treated with 100 μM morphine and 200 μMsinomenine for 48 h; (E) Astrocyte-derived exosomes were labeled by PKH67 and were taken up by neuronal SH-SY5Y cells.