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Folic acid had no toxic effect on RVECs. The cells (4000 per/well) were treated with NG or various concentrations of folic acid for 72 h. The abbreviation NG represents normal glucose, i.e., the cells were cultured in a medium with a glucose concentration at 5.5 mM. The effect of folic acid (0.1–0.001 μg/mL) on RVECs cytotoxicity was assessed by CCK-8 assay. The data was presented as means ± SD (n = 6).
