Table 2.
Thermocycling program for quantitative polymerase chain reaction.
| Step | Temperature, °C | Duration |
|---|---|---|
| 1 | 95 | 10 min |
| 2 | 95 | 15 sec |
| 3 | 60 | 60 sec |
| Steps 2–3 | - | 40 cycles |
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