Skip to main content
. 2018 Oct 26;11(1):1536192. doi: 10.1080/20002297.2018.1536192

Figure 1.

Figure 1.

SDS-PAGE analysis of outer membrane protein, and outer membrane vesicle preparations obtained from A. actinomycetemcomitans strains. OMP preparations were analyzed using Coomassie-staining (a), and Western blot using a polyclonal antiserum specific for E. coli OmpA (c). OMV preparations were analyzed using Silver-staining (b), and Western blot using a polyclonal antiserum specific for E. coli OmpA (d), or an A. actinomycetemcomitans-responsive serum from a periodontitis subject (e). Samples from the following strains are shown in the panels: D7SS (lane 1), D7SS omp100 ompA1 (lane 2 in panel a–d), D7SS ompA1 (lane 3), D7SS ompA1 R1 (lane 4), D7SS ompA1 ompA2 (lane 5), and D7SS omp100 (lane 2 in panel e). Selected protein bands are indicated with arrows. Sizes (kDa) of the proteins in the pre-stained molecular weight marker (M) are indicated along the left side.