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. 2018 Sep 11;23(9):2315. doi: 10.3390/molecules23092315

Figure 6.

Figure 6

Effect of fatty acids on the insulin-induced signaling cascade in HepG2 cells. HepG2 cells were treated 24 h with 0.25 mM PALM, C6, C8, C10, a C8 + C10 equimolar mix, or vehicle control (BSA) then stimulated (+), or not (−), with 100 nM insulin for 10 min. (a) Representative Western blots of total and phosphorylated Akt (Ser473), total and phosphorylated mTOR (Ser2448), and Cyclophilin-B loading control (n = 4). (b) Quantification of phosphorylation levels normalized to total levels. * p-value < 0.05, ** p-value < 0.01 < 0.001 versus non-insulin stimulated. # p-value < 0.05 versus insulin-stimulated BSA control. & p-value < 0.05 versus non-insulin stimulated BSA control.