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. Author manuscript; available in PMC: 2019 Oct 4.
Published in final edited form as: Cell Stem Cell. 2018 Sep 20;23(4):501–515.e7. doi: 10.1016/j.stem.2018.08.008

Figure 2:

Figure 2:

Anterior foregut spheroids have esophageal-respiratory competence.

(A) Schematic depicting experimental protocol to pattern AFG spheroids along the dorsal-ventral axis. (B) Current simplified model of the cues guiding dorsal-ventral patterning of the AFG of mouse and frog embryos. (C-G) qPCR analysis of 3-day-old spheroids (day 9) treated for 3 days with Noggin, untreated (ctrl), or chiron and BMP4 (10ng/mL) using dorsal markers SOX2 and MNX1 (C+E), the respiratory marker NKX2–1 (D), ΔN splice variant of TP63 (F), and the stratified squamous epithelium marker KRT4 (G). (H-I) IF staining for SOX2, NKX2–1, CDH1, and nuclei (DAPI) in Noggin (H) versus chiron+BMP4 (I) treated spheroids. Scale bar = 25μm. See quantification and statistical analysis section for details. See also Figure S3.