Table 1.

Trilineage differentiation scoring rubric for all lineages. Chondrogenesis rubric based on the Bern Score [15]

Lineage	Score
Chondrogenesis
Uniformity and darkness of stain
No stain	0
Weak staining of poorly formed matrix	1
Moderately even staining	2
Even dark stain	3
Distance between cells/amount of matrix accumulated
High cell densities with no matrix between	0
High cell densities with a little matrix in between	1
Moderate cell density with matrix	2
Low cell density with a moderate distance between	3
Cell morphologies represented
Condensed/necrotic/pycnotic bodies	0
Spindle/fibrous	1
Mixed spindle/fibrous with rounded chondrogenic morphology	2
Majority rounded/chondrogenic	3
Adipogenesis
0% of cells with stain uptake	0
1–25% of cells with stain uptake	1
26–50% of cells with stain uptake	2
51–75% of cells with stain uptake	3
76–100% of cells with stain uptake	4
Osteogenesis
0% of cells with stain uptake	0
1–25% of cells with stain uptake	1
26–50% of cells with stain uptake	2
51–75% of cells with stain uptake	3
76–100% of cells with stain uptake	4