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. 2018 Nov 8;9:294. doi: 10.1186/s13287-018-1014-y

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — FE promotes endothelial cell proliferation, migration, and tube formation. a HUVECs treated with FE at indicated concentrations. Cell proliferation assessed using cell counting kit, and percentage of optical density values relative to control calculated. b HUVEC migration evaluated using cell migration assay. c Percentage of gap closure (24 h) quantified. d HUVECs added to solidified Matrigel in a serum-free medium in presence or absence of FE. VEGF 20 ng/ml used as positive control. After 6-h incubation, endothelial cell tube formation stained using Calcein-AM and assessed by fluorescence microscopy. e Assessment of number of branch points/mm² in each group. f Quantification of mean tube length. *p < 0.05. VEGF vascular endothelial growth factor