Fig. 4.

(a) HCC827 cells were transfected as indicated. 72 h post transfection the cells were starved in serum free medium for 3 h. Whole cell lysates were harvested and levels of phosphorylated Erk1/2, Akt, Src and EGFR were assessed by immuno-blotting using anti-pERK1/2, anti-pAkt, anti-pSrc and, anti-pEGFR (Tyr1068). Total protein levels of Erk, Akt, Src and EGFR were also assessed in addition to α-tubulin, utilising Erk1/2, Akt, Src, EGFR and anti-tubulin antibodies. (b) H1975 cells were transfected as indicated. 72 h post transfection the cells were starved in serum free medium for 3 h. Whole cell lysates were harvested and levels of phosphorylated Erk1/2, Akt, Src and EGFR were assessed as before. Total protein levels of Erk, Akt, Src and EGFR were also assessed in addition to α-tubulin, as before