Table 1.
Primers used for MSP-PCR and real-time PCR
| Genes | Primer sense (5′–3′) | Primer antisense (5′–3′) | |
|---|---|---|---|
| BCL2L11 | M | AGTATTTTCGGTAAATAATGGGGT | GAATAAATCAAAAACTCCCAACG |
| U | GTATTTTTGGTAAATAATGGGGTTG | CAAATAAATCAAAAACTCCCAACA | |
| DAPK1 | M | GGATAGTCGGATCGAGTTAACGTC | CCCTCCCAAACGCCGA |
| U | GGAGGATAGTTGGATTGAGTTAATGTT | CAAATCCCTCCCAAACACCAA | |
| APAF1 | CCCAGAGGCTTCCACTTAATATTG | CAAACATCATCCAAGATCAAGAGAGA | |
| BAX | TGAGTACTTCACCAAGATTGCCA | AGTCAGGCCATGCTGGTAGAC | |
| BCL2L11 | TGTCTGACTCTCTCCGGACTG | TGACCACATCGAGCTTTAGCCAGTCA | |
| BNIP3 | CCAGAACATCATCCCTGCAT | TTCCAGTAGGGTCTCGACTTG | |
| BNIP3L | CTCAGGATCCACAGCAAACA | CCAGACTGGACTCTGCCTTC | |
| DNMT1 | CCATCAGGCATTCTACCA | CGTTCTCCTTGTCTTCTCT | |
| DNMT3a | TATTCATGAGCGCACAAGAGAGC | GGGTGTTCCAGGGTAACATTGAG | |
| STAT3 | CACCTTGGATTGAGAGTCAAGAC | AGGAATCGGCTATATTGCTGGT | |
M methylated, U unmethylated