Fig. 3.

Serotonin induces AKT/mTOR-independent autophagy and activates Notch signaling. a HepG2 and SK-Hep1 cells were treated with high (0.5 and 1 mM) and low (0.5 and 1 μM) concentrations of serotonin for 24 h. Cells were lysed and 50 μg proteins were immunoblotted with anti-phospho-Serine-473-AKT, anti-total AKT, anti-phospho-Serine-2448-mTOR, anti-total mTOR, anti-LC3β I/II, anti-ATG3, anti-phospho-Serine-65-4EBP1, anti-Beclin1 and anti-GAPDH antibodies. b HepG2 and SK-Hep1 cells were treated with indicated concentrations of serotonin for 24 h. Cells were washed with PBS, lysed with cell lysis buffer and 50 μg proteins were immunoblotted with anti-cleaved-Notch1-V1744, anti-Notch2, anti-Notch3, anti-Jagged1, anti-Hes1 and anti-GAPDH antibodies