Fig. 2.

Permeability barrier function test of urothelial differentiation of USC. a In vitro permeability test model of UC-induced USC in dynamic culture condition vs. static culture condition. b Urothelial induction of USC in different conditions of differentiation culture for 2 weeks. c Effect of time-dependent induction on urothelial differentiation of USC on weeks 1 and 2, respectively. d Impact of dynamic culture on permeability barrier function of USC 2 weeks after urothelial induction. Notes: Foot numbers in graphs c and d represent: (1) USC, (2) UC, (3) USC + UC/CM, (4) USC + EGF, (5) USC + SMC/CM. Abbreviations: USC = urine-derived stem cells, UC = urothelial cells, SMC = smooth muscle cells, CM = conditioned medium, UC/CM = urothelium-conditioned medium SMC/CM = smooth muscle cell-conditioned medium EGF = epidermal growth factor. Single seeding = seeding cells only once, Triple Seeding = seeding cells each at first 3 days