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. 2018 Nov 8;9:304. doi: 10.1186/s13287-018-1035-6

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — UC-induced USC expressing urothelial cell gene and protein markers. Expression level of urothelial cell transcripts and protein markers (Uroplakin Ia/III, AE1/AE3, and CK20) under different induction culture conditions assessed by: a real-time PCR, b Western blotting, and c immunofluorescence staining 14 days after urothelial differentiation. Cell morphology of USC, UC, and induced USC are also illustrated. Notes: Foot numbers in graphs (a) and (b) represent: (1) USC, (2) UC, (3) USC + UC/CM, (4) USC + EGF, (5) USC + SMC/CM. Abbreviations: UPIa = Uroplakin Ia, UPIII = Uroplakin III. USC = urine-derived stem cells, UC = urothelial cells, SMC = smooth muscle cells, CM = conditioned medium, UC/CM = urothelium-conditioned medium SMC/CM = Smooth muscle cells conditioned medium EGF = epidermal growth factor. Scale bar = 50 μm