Figure 2.

Intact GR dimerisation in stromal cells is crucial for proper anti-inflammatory glucocorticoid (GC) actions in serum transfer-induced arthritis (STIA). (A) Ankle thickness and (B) clinical score of wt mice and mice with an impaired GR dimerisation (GR^dim) treated with PBS (black and red) or DEX (grey and blue). (C) AUC for the clinical score of wt and GR^dim mice treated with PBS (black and red) or DEX (grey and blue). (D) H&E staining of paraffin sections of hind paws of wt and GR^dim mice (scale bar=200 µm); asterisks show area of inflammation. Group size n=5–7 mice per group. (E) Scheme of bone marrow transplantation of irradiated wt and GR^dim recipient mice that were reconstituted with wt bone marrow cells (wt→wt and wt→GR^dim). (F) Ankle thickness and (G) clinical score of mice treated with PBS (black and red) or DEX (grey and blue). (H) AUC of the clinical score of mice treated with PBS (black and red) or DEX (grey and blue). (I) H&E staining of paraffin sections of hind paws of wt→wt and wt→GR^dim mice (scale bar=200 µm); asterisks show area of inflammation. Group size n=4–6 mice per group. AUC, area under the curve; DEX, dexamethasone; GR, glucocorticoid receptor; ns, not significant; PBS, phosphate buffered saline; wt, wild type.