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. 2018 Nov 5;11:7853–7864. doi: 10.2147/OTT.S180131

Figure 2.

Figure 2

Knockdown of EZH2 overcame 5-FU resistance of GC cells.

Notes: (A, B) The cell viability was determined by MTT assay in AGS/5-FU and SGC-7901/5-FU cells and their parental cells exposed to different concentrations of 5-FU (0.1, 1, 5, 10, 25, 50, 100 µg/mL) for 48 hours. (C, D) qRT-PCR and Western blot analyses were performed in AGS/5-FU and SGC-7901/5-FU cells transfected with EZH2 siRNAs (si-EZH2 #1, si-EZH2 #2 or si-EZH2 #3) or si-con. (E, F) AGS/5-FU and SGC-7901/5-FU cells transfected with si-EZH2 #1 or si-con were treated with various concentrations of 5-FU (0.1, 1, 5, 10, 25, 50, 100 µg/mL) for 48 hours and cell viability was evaluated by MTT assay. (G, H) Cell apoptosis was determined by flow cytometry analysis in si-EZH2 #1 or si-con transfected AGS/5-FU and SGC-7901/5-FU cells after treatment with 30 µg/mL of 5-FU. *P<0.05.

Abbreviations: 5-FU, 5-fluorouracil; EZH2, enhancer of zeste homologue 2; GC, gastric cancer; qRT-PCR, quantitative real-time PCR; si-con, negative control.