Fig. 2.
DHE intensity is strongest in undifferentiated prohemocytes and in crystal cells. (A) Examples of hemocyte pairs showing inverse correlation between DHE fluorescence intensity and expression level of hemocyte GAL4 drivers Peroxidasin (Pxn), Hemese (He), and HemolectinΔ (HmlΔ). (B) Quantification of relationship between DHE fluorescence intensity and level of Pxn>GFP expression. Pearson correlation coefficient and P value calculated using values to the right of dotted line (Pxn-GFP > 400). (C) Examples of DHE fluorescence in crystal cells expressing Lozenge (Lz)-GAL4 and UAS-GFP (crystal morphology less distinct in aqueous media used for live imaging). (D,E) Comparison of DHE intensities (D) and cell body diameters (E) among Pxnpos hemocytes, Pxnneg hemocytes, and Lzpos crystal cells. Scale bars: 5μm.