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. Author manuscript; available in PMC: 2019 Nov 2.
Published in final edited form as: Biochem Biophys Res Commun. 2018 Oct 3;505(3):726–732. doi: 10.1016/j.bbrc.2018.09.126

Fig. 4.

Fig. 4.

ROS promote JNK-dependent crystal cell rupture and melanization. (A) Examples of crystal cells at 4 stages according to key. (B) Crystal cell stages 30 minutes after bleeding into 10 mM NAC, 2μM menadione, and 2μM menadione + 50μM SP600125. (C) Comparison of crystal cell expression of JNK transcriptional reporter TRE-Red after 30 mins incubation in 10 mM NAC, 2μM menadione, and 2μM menadione + 50μM SP600125. (D) Melanization (black) 40 minutes after 10 larvae each bled into 60 μM of Schneider’s media, 2 μM menadione, or 5 mM NAC. Scale bars: 10μm in (A) and 500μm in (D).

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