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. Author manuscript; available in PMC: 2019 Sep 6.
Published in final edited form as: Mol Cell. 2018 Aug 23;71(5):761–774.e5. doi: 10.1016/j.molcel.2018.07.028
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Mouse Monoclonal Antibody c-Myc (9E10) Roche 1167203001
Rabbit Polyclonal Antibody GCD6 Covance (CM002) (Bushman et al., 1993)
Rabbit Polyclonal Anti-Histone H3 Antibody Abcam Ab1791
Bacterial and Virus Strains
DH5alpha NEB C2987H
Rosetta pLacI-RARE Novagen 71404
Biological Samples
N/A N/A N/A
Chemicals, Peptides, and Recombinant Proteins
Cycloheximide Sigma C7698
RNase I Ambion AM2294
PNK NEB M0201L
Universal miRNA cloning linker NEB S1315S
T4 RNA ligase 2, truncated NEB M0242L
Superscript III Reverse Transcriptase Invitrogen 18080044
Lyticase Sigma L2524
Creatine kinase Sigma 000000010127566001
Creatine phosphate Sigma 10621714001
RiboLock RNase Inhibitor Thermo Fisher Scientific EO0381
D-luciferin (Beetle Luciferin, Potassium Salt) Promega E1602
DNase I recombinant, RNase-free Roche 04-716-728-001
SuperScript III First-Strand Synthesis SuperMix for qRT-PCR Invitrogen 11752-050
Critical Commercial Assays
QuikChange Lightning Site-Directed Mutagenesis Kit Agilent Technologies 210518
CircLigase ssDNA Ligase Kit Epicentre CL4115K
Agilent High Sensitivity DNA Kit Agilent 5067-4626
RiboMAX Large Scale RNA Production Systems Promega P1300
Vaccinia Capping System NEB M2080S
Brilliant III Ultra-Fast SYBR Green QPCR Master Mix Agilent Technologies 600882
Deposited Data
Raw and analyzed data This paper GEO: GSE108942
Experimental Models: Cell Lines
N/A N/A N/A
Experimental Models: Organisms/Strains
A full list of yeast strains used in this study is provided in Table S1. This paper N/A
Oligonucleotides
A full list of oligonucleotides used in this study is provided in Table S2. This paper N/A
Recombinant DNA
A full list of plasmids and high copy reporter plasmids used in this study are provided in Tables S3 and S4. This paper N/A
Software and Algorithms
Igor Pro Version 7.00 Wavemetrics 15-500
ImageJ Version 2.00-rc-43/1.51p https://imagej.nih.gov/ij/ N/A
Position average and metagene This paper makeposavg makeavggene
Bowtie Github (Langmead et al., 2009)
Other