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In vitro confirmation of the 12-marker panel in activated LX2.
Notes: (A) Immunocytofluorescence of LX2, (B) quantitative real-time PCR determination of the 12 markers in the LX2 (Huh7, LX2 activated by Huh7 conditioned cultured medium; NC, RPMI-1640 treated LX2 as the negative control; *P<0.01; **P<0.001).
Abbreviations: α-SMA, α-smooth muscle actin; NC, negative control.
