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Levels of the tight junction proteins claudin-1 [A], claudin-5 [B], and tricellulin [C] assessed by immunofluorescence in ileal biopsies. [D] Representative photographs of claudin-5 staining in one twin with Crohn’s disease [CD], the healthy co-twin, and the external control. The sealing tight junction protein claudin-5 [red] was mostly expressed on the apical side of the colonocytes; blue = staining of cell nucleus with DAPI. Comparisons were done using the Mann–Whitney U test.
