Figure 7. Reaction of DKG with Fenton mixture.

Non-radioactive DKG [50 mM in 0.1 M acetate (Na⁺, pH 4.8)] was incubated with Fenton reagent (1 mM each of EDTA, FeSO₄, and H₂O₂) for up to 30 min, after which further ^•OH action was stopped by the addition of ethanol (to 50%). This DKG was prepared by the potassium iodate method. The time-0 sample represents DKG with ethanol added instantly after the Fenton reagents. Samples were electrophoresed at pH 2.0 and the products stained in AgNO₃. The position of internal marker orange G is circled in pencil.