Figure 8. Reaction of AA catabolites with singlet oxygen.

DHA at 50 mM in 0.1 M acetate (a,b) or DKG at 25 mM in 0.16 M acetate (c) (both buffers Na⁺, pH 4.8) was incubated with 1 mM riboflavin in the light (which generates ¹O₂) or in darkness. Samples taken at 0–24 h were electrophoresed at pH 6.5 (a,c) or 2.0 (b). Orange G (internal marker) was circled in pencil, then the metabolites were stained with AgNO₃.