. Author manuscript; available in PMC: 2018 Nov 9.

Published in final edited form as: Mol Microbiol. 2016 Sep 27;102(5):850–864. doi: 10.1111/mmi.13495

Table 3.

Bacterial strains and plasmids used in this study.

Strain/Plasmid	Description^a	Source or reference
*S. aureus*
USA300	USA300 LAC cured of antibiotic resistance plasmid	(Arsic et al., 2012)
RN4220	$r_{K}^{-} m_{K}^{-}$ ; capable of accepting foreign DNA	(Kreiswirth et al., 1983)
bcaP	USA300 bcaP∷ΦNΣ; Em^R (SAUSA300_2538)	(Fey et al., 2013)
brnQ1	USA300 ΔbrnQ1	(Kaiser et al., 2015)
brnQ1-bcaP	USA300 ΔbrnQ1 bcaP∷ΦNΣ; Em^R	This study
brnQ1-2-3	USA300 ΔbrnQ1 ΔbrnQ2 brnQ3∷Tc; Tc^R	(Kaiser et al., 2015)
brnQ1-2-3-bcaP	USA300 ΔbrnQ1 ΔbrnQ2 brnQ3∷Tc bcaP∷ΦNΣ; Em^R, Tc^R	This study
brnQ1-2-bcaP	USA300 ΔbrnQ1 ΔbrnQ2 bcaP∷ΦNΣ; Em^R	This study
brnQ1-3-bcaP	USA300 ΔbrnQ1 brnQ3∷Tc bcaP∷ΦNΣ; Em^R, Tc^R	This study
codY	USA300 codY∷ ΦNΣ; Em^R	(Fey et al., 2013)
crtM	USA300 crtM∷ ΦNΣ; Em^R	(Fey et al., 2013)
brnQ1-crtM	USA300 ΔbrnQ1 crtM∷ ΦNΣ; Em^R	This study
*E. coli*
DH5α	F⁻ ΅80dlacZΔM15 recA1 endA1 gyrA96 thi-1 hsdR17 ( $r_{K}^{-} m_{K}^{-}$ ) supE44 relA1 deoR Δ(lacZYA-argF)U169phoA	Promega
Plasmids
pRMC2	Anhydrotetracycline-inducible expression vector; Ap^R in E. coli; Cm^R in S. aureus	(Corrigan and Foster, 2009)
pBrnQ1	pRMC2 containing brnQ1; Cm^RpRMC2 containing bcaP; Cm^R	(Kaiser et al., 2015)
pBcaP	Vector harboring promoterless luxABCDE operon; Cm^R	This study
pGYlux	lux reporter vector with brnQ1 promoter	(Mesak et al., 2009)
pGYbrnQ1∷lux	lux reporter vector with bcaP promoter	This study
pGYbcaP∷lux		This study

^a.

Abbreviations: Tc^R, Cm^R, Em^R and Ap^R designate resistance to tetracycline, chloramphenicol, erythromycin and ampicillin respectively.