FIGURE 5.

Setd7 knockdown increased the DNA damage following oxidative stress induced by H₂O₂. (A) LNCaP cells were treated with H₂O₂ at 50 and 400 μM, and then, the cells were harvested and analyzed to determine the Setd7, Nrf2, Nqo1 and Gstt2 mRNA levels using qPCR; (B) the levels of DNA damage in LNCaP cells induced by 100 μM H₂O₂ were measured with an alkaline comet assay. Representative images of the comet assays to assess DNA integrity; (C) parameters tested for DNA damage. The mean values and standard deviations represent triplicate samples. The data shown are representative of three experiments. * The difference was statistically significant when p < 0.05 compared with the control cell results.