Figure 3.
(A–B) The combined treatment of JQ1 and trametinib promoted tumor regression in anaplastic thyroid tumors in athymic mice. Equal numbers of THJ-11T cells (5 × 106) or THJ-16T cells (5 × 106) were injected into the flanks of mice before treatment. When tumors reached the size of 200 mm3, vehicle, JQ1, or trametinib was administered for 10 days. Tumor growth curves (I), tumor size (II) and tumor weight (III) for THJ-11T (A) and THJ-16T (B) are shown (n = 5 for each treatment). Ki67 staining of sections of tumor derived fromTHJ-11T cells (C-I) or from THJ-16T cells (D-I) as described in Methods. The treatment for each panel is marked. The Ki67 positively-labeled cells were counted, and % of Ki-67-positive cells is graphed in (C-II and D-II). The p values are indicated. (E-I and F-I). The H & E slides were prepared according to Methods. Representative pathohistological characteristics of xenograft tumors of ATC cells treated with vehicle (a, c, e) or with combined treatment (b, d, f) are marked. (E-II and F-II). % occurrence of capsular thickness (panel a), capsular invasion (panel b), and large nucleoli (panel c) were quantitified from H & E stained sections derived from THJ-11T cells (E-II, n = 5) and THJ-16T cells (F-II, n = 5). # denotes not detectable.