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. 2018 Oct 23;9(83):35408–35421. doi: 10.18632/oncotarget.26253

Figure 6. The combined treatment of JQ1 and trametinib synergistically decreased the MYC mRNA expression in ATC cell lines and tumors.

Figure 6

The mRNA level of the MYC gene was measured by real-time RT–PCR in THJ-11T cells (A-I) and in THJ-16T cells (B-I). The mRNA level of the MYC gene was measured by real-time RT–PCR in the THJ-11T xenograft tumors (A-II) and in the THJ-16T tumors (B-II). PCRs were performed using 200 ng of total RNA. GAPDH was used as a reference. The combined treatment of JQ1 and trametinib synergistically reduced the recruitment of BRD4 on the promoter of the MYC gene in THJ-11T cells (C-I) and in THJ-16T cells (C-II). ChIP assay was carried out using noremal rabbit IgG or BRD4 antibody. The experiments were carried out in triplicates. The p values are shown (n = 3).