Figure 3. A_2AR disruption exacerbates the effects of HFD feeding on inducing weight again, adiposity, and hyperglycemia.

Male A_2AR^−/−, A_2AR^+/−, and A_2AR^+/+ mice, at 5 - 6 weeks of age, were fed an HFD for 12 weeks. (A) Cumulative food intake during feeding period. (B) Body weight was measured before and after the feeding period. (C) Abdominal fat mass was calculated as the sum of epididymal fat, mesenteric fat, and perinephric fat. Adiposity was calculated as the ratio of abdominal fat mass to body weight. (D) After the feeding period, mice were fasted for 4 hr prior to collection of blood samples. Plasma levels of glucose were quantified using a metabolic kit. For A - D, data are means ± SEM. n = 4 - 6 (A) or 10 – 12 (C - D). Statistical difference between A_2AR^−/− and A_2AR^+/+: *, P < 0.05 and **, P < 0.01 in B after feeding period or in C and D; statistical difference between A_2AR^−/− and A_2AR^+/−: ^†, P < 0.05 in B after feeding period or in D; statistical difference between A_2AR^+/− and A_2AR^+/+: ^‡, P < 0.05 ^‡‡, P < 0.01 in B after feeding period or in C and D.